Introduction of SELEX and Important SELEX Variants / Yiyang Dong, Zhuo Wang, Sai Wang, Yehui Wu, Yufan Ma, Jiahui Liu -- In Chemico Modification of Nucleotides for Better Recognition / Przemyslaw Jurek, Marta Matusiewicz, Maciej Mazurek, Filip Jelen -- Immobilization of Aptamers on Substrates / Annalisa De Girolamo, Maureen McKeague, Michelangelo Pascale, Marina Cortese, Maria C DeRosa -- Characterization of Aptamer-Ligand Complexes / Rebeca Miranda-Castro, Noemí de-los-Santos-Álvarez, María J Lobo-Castañón -- Utilization of Aptamers for Sample Preparation in Analytical Methods / Zhiyong Yan, Yang Liu -- Development of Aptamer-Based Colorimetric Analytical Methods / Subash CB Gopinath, Thangavel Lakshmipriya, MK Md Arshad, Chun Hong Voon -- Enzyme-Linked Aptamer Assay (ELAA) / Yiyang Dong, Sai Wang -- Development of Aptamer-Based Fluorescence Sensors / Seyed M Taghdisi, Rezvan Yazdian-Robati, Mona Alibolandi, Mohammad Ramezani, Khalil Abnous -- Development of Aptamer-Based Electrochemical Methods / Jian-guo Xu, Li Yao, Lin Cheng, Chao Yan, Wei Chen -- Development of Aptamer-Based Lateral Flow AssayMethods / Miriam Jauset-Rubio, Mohammad S El-Shahawi, Abdulaziz S Bashammakh, Abdulrahman O Alyoubi, Ciara K O'Sullivan -- Development of Aptamer-Based Non-labeling Methods / Huajie Gu, Liling Hao, Zhouping Wang -- Challenges of SELEX and Demerits of Aptamer-Based Methods / Haiyun Liu, Jinghua Yu -- State of the Art and Emerging Applications / Lin-Chi Chen, Jui-Hong Weng, Pei-Wei Lee.

Includes bibliographical references and index.

Online resource; title from PDF title page (EBSCO, viewed October 22, 2018).

Adopting an analyst and developer-centered approach, this comprehensive monograph investigates in detail analytical method development and improvements with established systems and beyond. In recent years, aptamer-based systems have been developed for a wide-range of analytical and medical applications. This book offers an introduction to the topic, outlines the common protocols for aptamer synthesis, as well as providing information on the different optimisation strategies that can obtain higher affinities to target molecules. The contributors provide an in-depth review of the characterisation of aptamer-target molecule interaction and immobilisation strategies and discuss the developments of methods for all the relevant applications.